Journal: bioRxiv
Article Title: Paper-based Sensing of Fucosylated Biological Compounds
doi: 10.1101/2020.10.30.362889
Figure Lengend Snippet: (A) Reaction with free fucose (left) and fucosylated HMOs, 2’FL and 3-FL (right). Assays were performed with 500 mg/L of L-fucose, 2’-FL and 3-FL (B) A 25-reaction array with two different linkage specific fucosidases in different combinations. Image was captured after the paper was dry. (C) Transfer function for sensing 2’-FL. Response curve depicts mean color intensity (analyzed in triplicate). The dose-response curve was fitted with a 4PL model, shown by the line. The top shows a capture of the signal expression from increasing concentrations of 2’-FL. (D) Calibration curve for 3-FL, as described in (C). Error bars represent standard deviation of triplicate measurements of mean color intensities.
Article Snippet: The experimental data were fitted into a 4-parameter logistic model (4PL) using MATLAB and the resulting transfer functions revealed the dynamic range and limits of detection.
Techniques: Expressing, Standard Deviation